Non-structural protein NS4B: HCV replication web inducer
نویسندگان
چکیده
منابع مشابه
Analysis of Immumoreactivity of Heterologously Expressed Non-structural Protein 4B (NS4B) from Hepatitis C Virus (HCV) Genotype 1a
Background: Detection of hepatitis C virus specific antibodies is the initial step in chronic HCV diagnosis. HCV NS4B is among the most immunogenic HCV antigens and has been widely used in commercial Enzyme Immunoassays (EIA). Additionally, NS4B, a key protein in the virus replication, can be an alternative target for antiviral therapy. Objectives: Development of a new method for high-level ex...
متن کاملanalysis of immumoreactivity of heterologously expressed non-structural protein 4b (ns4b) from hepatitis c virus (hcv) genotype 1a
background: detection of hepatitis c virus specific antibodies is the initial step in chronic hcv diagnosis. hcv ns4b is among the most immunogenic hcv antigens and has been widely used in commercial enzyme immunoassays (eia). additionally, ns4b, a key protein in the virus replication, can be an alternative target for antiviral therapy.objectives: development of a new method for high-level exp...
متن کاملA nucleotide binding motif in hepatitis C virus (HCV) NS4B mediates HCV RNA replication.
Hepatitis C virus (HCV) is a major cause of viral hepatitis. There is no effective therapy for most patients. We have identified a nucleotide binding motif (NBM) in one of the virus's nonstructural proteins, NS4B. This structural motif binds and hydrolyzes GTP and is conserved across HCV isolates. Genetically disrupting the NBM impairs GTP binding and hydrolysis and dramatically inhibits HCV RN...
متن کاملThe Roles of Endoplasmic Reticulum Overload Response Induced by HCV and NS4B Protein in Human Hepatocyte Viability and Virus Replication
Hepatitis C virus (HCV) replication is associated with endoplasmic reticulum (ER) and its infection triggers ER stress. In response to ER stress, ER overload response (EOR) can be activated, which involves the release of Ca2+ from ER, production of reactive oxygen species (ROS) and activation of nuclear factor κB (NF-κB). We have previously reported that HCV NS4B expression activates NF-κB via ...
متن کاملGene expression profiles of HeLa Cells impacted by hepatitis C virus non-structural protein NS4B.
By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RTPCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transien...
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ژورنال
عنوان ژورنال: Asian Pacific Journal of Tropical Disease
سال: 2012
ISSN: 2222-1808
DOI: 10.1016/s2222-1808(12)60111-x